MAbs:
IBP144, IBP182, IBP190New MAbs: IBP3, IBP154, IBP163, IBP180, IBP185Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cellsof Balb/c mice immunized with recombinant human IGFBP-4 expressed in mammalian cell line(MAbs IBP144, IBP182, IBP190, IBP180, IBP185, IBP154), synthetic peptide from human IGFBP-4His142- Met156 (MAb IBP3) or synthetic peptide from human IGFBP-4 Lys157-Gln171 (MAb IBP163).
Specificity:
MAbs IBP144, IBP154 and IBP180:human IGFBP-4; human N-terminal IGFBP-4 fragment (NT-IGFBP-4)MAbs IBP182, IBP185 and IBP190:human IGFBP-4; human C-terminal IGFBP-4 fragment (CT-IGFBP-4)MAb IBP3:human NT-IGFBP-4, cross-reaction with full-length human IGFBP-4MAb IBP163:human CT-IGFBP-4, cross-reaction with full-length human IGFBP-4 <5% (ELISA)
MAb Isotypes:
IgG1 for MAbs IBP163, IBP190IgG2a for MAbs IBP144, IBP154, IBP180 IgG2b for MAb IBP182, IBP185 IgG3 for MAb IBP3
Applications:
Detection of human IGFBP-4, NT-IGFBP-4, and CT-IGFBP-4. MAbs are working in direct ELISA and sandwich immunoassay.Recommended pairs for human IGFBP-4 sandwich immunoassay (capture-detection): IBP185 –IBP154 IBP182 – IBP144 IBP144 – IBP190 Recommended pairs for specific human NT-IGFBP-4 sandwich immunoassay (capture-detection):IBP3 – IBP144 IBP3 – IBP180 Recommended pairs for specific human CT-IGFBP-4 sandwich immunoassay (capture-detection): IBP182 – IBP163
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Material Safety Note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
