Recombinant NT-proBNP corresponds to the fragment 1-85 a.a.r. of canine proBNP and contains additional affinity tag sequence of 16 a.a.r. at the N-terminus.

Totally, recombinant NT-proBNP conists of 101 a.a.r.

Predicted molecular mass 10545 Da is confirmed by MALDI-MS.

Purity > 95% (Tricine-SDS-PAGE).

Concentration is determined spectrophotometrically.

Immunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of canine NT-proBNP molecule (Cat# 2-CNT).

10 mM potassium phosphate, pH 7.4, 150 mM NaCl.

-70 °C (-65 … -80 °C allowed)

Avoid repeated freezing and thawing. It is recommended to aliquot the product after the first thawing.

To prepare diluted samples of NT-proBNP it is recommended to use buffer solutions containing carrier protein and detergent (e.g. 0.05 M Tric-HCl, pH 7.8, 0.15 M NaCl, 0.01% Tween 40, 0.5% bovine serum albumin).

Canine NT-proBNP Assay Notes

Search NT-proBNP canine references from PubMed

Cat# 2-CNTMonoclonal mouse anti-canine N-terminal proBNP (NT-proBNP)