Advanced immunochemical/Human cardiac troponin I (cTnI), phosphorylated/8-TIc-ph,Advanced immunochemical,,Advanced immunochemical lnc,advimmuno,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Advanced immunochemical/Human cardiac troponin I (cTnI), phosphorylated/8-TIc-ph

产品编号：8-TIc-ph

市场价：¥47180.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>植物抗原>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$2359.00

品牌： Advanced immunochemical

公司：Advancedimmunochemical

公司分类：

商品介绍

General Category:

Antigens » Cardiac Markers » Troponin I and Troponin complex

Disease Category:

Cardiac Markers » Antigens » Troponin I and Troponin complex

Source:

Human heart tissue.Blood samples from the tissue donors were tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV, and syphilis.

Applications:

TnI is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium sensitivity to striated muscle actomyosin ATPase activity.

Cardiac isoform of TnI (cTnI) has two serine residues at positions 22 and 23 which could be phosphorylated by cAMP-dependent protein kinase (PKA) in response to β-adrenergic stimulation of the heart. Modification of these serines results in the changes of myocardial contractility. About 50% of cTnI purified from human cardiac tissue is mono- or biphosphorylated. cTnI purified from human cardiac tissue was completely phosphorylated in vitro by catalytic subunit of PKA from bovine heart. TnI is suitable for use as a standard in immunoassay, immunogen for antiserum production.

Analysis:

Purity > 95 %.TnI concentration was determined spectrophotometrically using A (0.1 %, 280 nm, 1 cm) equal to 0.42. This coefficient was calculated from the amino composition of human cTnI (FEBS Lett, 270, 57- 61).Phosphate incorporation confirmed by reaction with monoclonal antibody that doesn’t react with phosphorylated cTnI in ELISA and immunoblotting.

Presentation:

Lyophilized from 0.01 M HCl.Product is recommended to reconstitute with urea/Tris buffer, pH 7.5 (7 M urea, 5 mM EDTA, 15 mM mercapthoethanol, 20 mM Tris) to concentration around 1mg/ml.Solubility is limited at neutral pH and physiological salt concentration.

Storage Conditions:

-20 °C (between -15 and -30 °C allowed)-70 ºC for long term storage (between -65 and -80 °C allowed)

Material Safety Note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

References:

Search Troponin I references from PubMed. See all Troponin references.

Related Products:

See othercardiac markers andblood coagulation products.

品牌介绍

Advanced ImmunoChemical以其在研究和临床诊断市场上提供高质量的ISO-9001：2015单克隆和多克隆抗体，抗原，酶和纯化的蛋白质而著称。我们几乎所有的产品均已通过验证，可用于定量夹心免疫分析和定性快速检测。

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