MAbs:
HSP25-19, HSP25-31Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice, immunized with recombinant chicken HSP25.
Specificity:
HSP25/27Cross-reaction of anti-HSP25/27 MAbs with HSP25/27 from different animal species (Western blotting with extracts of cardiac tissue).
MAb Isotypes:
IgG2a for MAb HSP25-19IgG2b for MAb HSP25-31
Applications:
HSP25/27 detection in ELISA, Western blotting, immunofluorescent staining of cell cultures,HSP25/27 affinity purification and immunoprecipitation of HSP25/27 from cell/tissue extracts of different animals.
Purification:
Chromatography on protein G Sepharose
Presentation:
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Material Safety Note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.