Specificity:
MAbs 28A4, and 27E8 specifically recognize beta-subunit of HCG. MAbs are not cross-reacting with hLH, hTSH, hFSH.MAb 77F12 specifically recognizes HCG; it reacts mainly with alpha-subunit. MAb is cross-reacting with whole molecule of HCG, hLH, hTSH and hFSH. Cross-reactivity with free beta-subunit of HCG was not detected.MAb F1 is specific for the alpha-chain of FSH, LH, TSH and HCG.
MAb Isotypes:
IgG for MAb F1IgG1 for MAbs 27E8IgG2a for MAb 28A4IgG2b for MAbs 77F12
Applications:
Detection of HCG in immunoassays. Suitable for immunochromatography (not tested with MAb F1).Best recommended pairs for sandwich immunoassay (capture-detection):77F12-28A4, 27E8-28A4
Purification:
Ion exchange chromatography for MAb F1.Chromatography on protein A Sepharose for MAbs 28A4, 27E8 and 77F12.
Presentation:
0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% sodium azide (NaN3) for MAb F1.PBS, pH 7.4, 0.1 % sodium azide (NaN3) for MAbs 28A4, 27E8 and 77F12.
Material safety note:
This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.